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KMID : 0644120080210020108
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Journal of Oriental Gynecology
2008 Volume.21 No. 2 p.108 ~ p.124
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Proteomic Approach to Study the Antioxidant Activities of Dioscoreae Rhizoma on HeLa Cells
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Yang Jeong-Min
Lee Ji-Hyung
Sung Jung-Suk
Kim Dong-Il
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Abstract
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Purpose: This study was examined to verify the anti-oxidative effect of Dioscoreae Rhizoma on HeLa cells by proteomic approach.
Methods: Aqueous extract was used to treat HeLa cell with different concentrations treated with water or MeOH extract of Dioscoreae Rhizoma. HeLa cells were co-treated with H2O2 and Dioscoreae Rhizoma extracts. Proteomics was done to identify, characterize, and quantitate proteins expressed in HeLa cells treated by H2O2 and Dioscoreae Rhizoma.
Results: When HeLa cells were Co-treated with H2O2 and Dioscoreae batatas extracts, 16 proteins identified by 2-DE and MALDI-TOF mass spectrometry and database search. PRDX, HSP27 was major proteins of antioxidant effect by
Dioscoreae batatas.
Conclusion: Our results suggest that Dioscoreae Rhizoma extracts induce antioxidant effects by regulating proteins such as PRDX, HSP27.
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KEYWORD
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Dioscoreae Rhizoma, HeLa cell, antioxidant, Proteomics, 2-DE
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